click-it tm plus terminal deoxynucleotidyl transferase dutp nick-end labeling (tunel) assay  (Thermo Fisher)

Journal: Cell

Article Title: CRATER tumor niches facilitate CD8 + T cell engagement and correspond with immunotherapy success

doi: 10.1016/j.cell.2025.09.021

Figure Lengend Snippet: (A) Three examples of CRATERs in 3D confocal images of whole-mount TUNEL assay, in PBS- or CPG ODN-injected tumors (four times daily), WT or melanocyte-specific B2M KO. (B) TUNEL + cell density in CRATERs and non-CRATER tumor area. (Image quantification. n = PBS WT-3 fields of view [FOV] from 3 fish, CpG ODN WT-12 FOV from 8 fish, CpG ODN B2M KO-5 FOV from 2 fish. Whiskers represent 1.5× the interquartile range. t test. p values: *PBS vs. CpG ODN WT = 0.03, **CPG ODN: CRATER vs. tumor = 0.005, **CpG ODN: WT vs. B2M KO = 0.007). (C) 3D imaging of whole-mount TUNEL staining of CpG ODN-treated tumor. White dashed lines: CRATER. Rectangle area enlarged. Image slightly rotated. (D) CRATER coverage in tumors of cd8α:EGFP (WT), cd8α:EGFP;prkdc −/− , or jak3 −/− transgenic fish ( n fish = WT-5, prkdc (−/−) -2, jak3 (−/−) -3 fish. Mean ± SE. t test, p values: *WT/cd8a:EGFP;prkdc (−/−) = 0.011, * WT/jak3 (−/−) = 0.01). (E) Representative 3D confocal images of melanoma in WT Tg(cd8a:EGFP) and Tg(cd8a:EGFP;prkdc (−/−) ) fish. Arrows: CD8 + T cells. See also .

Article Snippet: Click-iT TM Plus TUNEL Assay Kit , Fisher Scientific , Cat #: C10619.

Techniques: TUNEL Assay, Injection, Imaging, Staining, Transgenic Assay

Journal: Bioactive Materials

Article Title: Application of bioactive materials primary cilia as a novel delivery vehicle

doi: 10.1016/j.bioactmat.2025.04.015

Figure Lengend Snippet: Cilia delivery vehicle can be used to promote cellular remission, improve blood flow and induce melanogenic cytotoxicity. a. Cilia delivery vehicle on cellular remission of the melanoma tumor. Tumor tissues are labelled with metastasis tumor marker CD31 (green) and proliferation marker Ki-67 (green). TUNEL (green) assay is also used on tumor tissues to show potential DNA fragmentation occurring in apoptotic cells. In all cases, DAPI (blue) is used to indicate cell nuclei. Scale bar = 50 μm. b. The expression of CD31 is quantified. c. The expression of Ki-67 is quantified. d. DNA breaks in terms of fluorescence intensity of TUNEL assay is quantified. Additional data for expression of CD31, Ki-67 and TUNEL are available in . a.u. = arbitrary unit (area under the curve); FI=Fluorescence Intensity of collected photons per mm 2 scanned area. e. Functional Doppler sonagrams (ultrasound imaging) show blood flow in the tumor. The velocity of blood flow in the tumor is measured within each sonagram by isolating each individual pulse as indicated by the blue lines (regions of interest; ROI). Representative videos are shown in the . f-h . Each ROI represents (f) peak systolic velocity (PSV) and (g) end-diastolic velocity (EDV) and (h) mean velocity of blood flow. Quantitative summary of the measurements is shown. e-f. H&E histology analysis is performed in the tumor tissues. The presence of melanosomes (i) and melanosome bursting (j) are quantified in randomly taken areas of tumor tissues (0.045 mm 2 ). Representative images for H&E histology study are available in . N = 5–6 for each group. ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001; ∗∗∗∗, p < 0.0001 compared to control group. #, p < 0.05; ##, p < 0.01; ###, p < 0.001; ####, p < 0.0001compared to DOX-only group.

Article Snippet: TUNEL assay was performed to examine the apoptosis in tumor tissues for all the groups using Click-iT™ Plus TUNEL Assay Kits (Thermofisher, Catalog No. C10617 ) following manufacturer's protocol.

Techniques: Marker, TUNEL Assay, Expressing, Fluorescence, Functional Assay, Imaging, Control